茶树新梢不同叶片中β-葡萄糖苷酶和β-樱草糖苷酶基因表达的实时定量PCR分析

doi:10.13305/j.cnki.jts.2006.01.002

Abstract

Abstract: β-glucosidase and β-primeverosidase are considered the most important genes related to made tea aroma. An absolute quantification method for gene expression in tea plant using real-time PCR analysis was established and, the expression of β-glucosidase and β-primeverosidase genes in different leaves of Longjing 43 young shoot were determined using this method. The results indicate that the fourth leaf of the ‘five and a bud’ shoot has the highest copy number of β-glucosidase mRNA, which is 2.86E+08 copies/μl, the 4^th leaf>the 3^rd leaf>the 5^th leaf>the 2^nd leaf>one and a bud. And the highest β-primeverosidase expression is in the one and a bud, corresponding to 4.31E+06 copies/μl, one and a bud>the 2^nd leaf>the 3^rd>the 4^th leaf> the 5^th leaf. The expression intensity and pattern of these genes, which are closely related to tea aroma, are different in the young shoot. The results illuminate that the new developed method could be effectively used to detect gene expression quantitatively in tea plant.

Key words: tea plant, β-glucosidase, β-primeverosidase, real-time PCR

CLC Number:

S571.1
Q523

ZHAO Li-ping, CHEN Liang, WANG Xin-chao, YAO Ming-zhe. Quantitative Detection of β-glucosidase, β-primeverosidase Gene Expressions in Different Leaves of Tea Plantby Real-Time PCR Analysis[J]. Journal of Tea Science, 2006, 26(1): 11-16.

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Quantitative Detection of β-glucosidase, β-primeverosidase Gene Expressions in Different Leaves of Tea Plantby Real-Time PCR Analysis

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