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Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (3): 172-180.doi: 10.13305/j.cnki.jts.2008.03.011

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Systematic Separation, Purification of Water-soluble Green Tea Polysaccharides and Their Immunological Activities

KOU Xiao-hong1,2, JIANG He-yuan1,*, CUI Hong-chun1,2, ZHANG Jian-yong1, GAO Qing-qing1,2, YUAN Xin-yue1, SHU Ai-min1, LIU Xiao-hui1,2, GAO Qi1   

  1. 1. Key laboratory of Tea Chemical Engineering, the Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agriculture Science, Hangzhou 310008, China;
    2. Chinese Academy of Agriculture Science, Beijing 100081, China
  • Received:2008-03-10 Revised:2008-04-09 Online:2008-06-15 Published:2019-09-16

Abstract: Water-soluble green tea polysaccharides were separated and purified by Ultrafiltration membranes with pore diameter of 150 kD, 20 kD, 6 kD in turn. All the three fractions were further separated and purified by DEAE-cellulose ion-exchange chromatography and Sephacryl S-300 column chromatography, and more than 20 green tea polysaccharide components totally were obtained. Their purity and molecular weight distribution were determined with high performance gel permeation chromatography (HPGPC) equipped with ELSD detector, and five components of homogeneous polysaccharide were obtained. The immunological activities of these green tea polysaccharides were determined by stimulated phagocytosis and nitric oxide production on RAW264.7 macrophage cell line of mice. Results showed that the tea Polysaccharides with 20 kD MW possessed significant immunological activities on NO production or phagocytosis of mice macrophage Raw 264.7 cells.

Key words: tea polysaccharide, ultrafiltration, seperation, purification, immunologic activity

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