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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (2): 156-164.doi: 10.13305/j.cnki.jts.2014.02.009

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Study on the Inhibition Effects of EGCG on the Expression of RCCs-RAGE Axis in Diabetic Mouse Induced by Alloxan Monohydrate

ZHONG Yuan1, ZHANG Jing1, LIU Zhonghua1,2,3,*, CAI Shuxian1,3,*, LUO Guoan4, HUANG Jian′an1,2, WU Xianglan1, QU Shaoming2, JIN Lisha1   

  1. 1. Key Laboratory of Ministry of Education for Tea Science,Hunan Agricultural University, Changsha 410128,China;
    2.National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Basic Medical College of Peking University Health Science Center, Beijing 100191, China;
    4. Department of Chemistry of Tsinghua University and Key Laboratory of Biological Organic Phosphorus and Chemical Biology of Ministry of Education, Beijing 100084, China
  • Received:2013-05-08 Revised:2013-07-15 Online:2014-04-15 Published:2019-09-03

Abstract: The RCCs-RAGE signal axis induced by RCCs, including AGEs and 4-HNE, plays a key role in degenerative diseases, such as diabetes and cancer. To study on inhibiting activity effects of EGCG on RCCs-RAGE, diabetic mouse induced by intraperitoneal injection Alloxan monohydrate were divided into model group, EGCG-L (10 mg·kg-1·d-1) group, EGCG-M (20 mg·kg-1·d-1) group, and EGCG-H (30 mg·kg-1·d-1) group according to body weight and blood glucose. After 12 days’ administration respectively to three groups of diabetic mouse by gavage, blood glucose value, insulin, sRAGE concentration, carbonyl content and fluorescence value of AGEs in serum were determinde, while the expressions of RAGE gene in kidney were detected by QPCR, RAGE protein and 4-HNE experiment by western bolt. Result showed, compared to model group, EGCG could improve diabetes symptom of mouse by significantly decreasing the formation of RCCs, including carbonyl, 4-HNE and AGEs, increasing the concentration of sRAGE in serum, inhibiting the inflammation reaction induced by RCCs-RAGE, and alleviating the oxidation stress effectively. The study revealed that EGCG inhibited the RCCs-RAGE signal axis may be one of the potential mechanisms in the treatment of degenerative diseases.

Key words: EGCG, diabetes, reactive carbonyl compounds, receptor for advanced glycation end products

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