Subcellular Localization of CsE1&#x003b1; as well as Cloning and Expression of Its Promoter from the Pollen of Camellia sinensis

DU Yulin; WANG Weidong; WANG Yuhua; LI Xinghui

doi:10.13305/j.cnki.jts.2015.03.012

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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (3) : 290-298. DOI: 10.13305/j.cnki.jts.2015.03.012

Subcellular Localization of CsE1α as well as Cloning and Expression of Its Promoter from the Pollen of Camellia sinensis

DU Yulin, WANG Weidong, WANG Yuhua^*, LI Xinghui

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Abstract

In this paper, full-length cDNA is identified for designing the gene-special primers in TAIL-PCR to clone the promoter of CsE1α. By sequencing and bioinformatic analyzing, we observed two CAAT-box, two TATA-box, two GATA-box, one LTR and one G-box located in the 336βbp promoter region. After constructing and transferring the transient expression vectors into the onion epidermal cells, subcellular localization of CsE1α-GFP fusion proteins is verified in mitochondria, while the promoter can activate downstream gene expressing in entire cell. This experiment may provide useful information for the subsequent stable expression in transgenic Arabidopsis and the further investigation on the expression and regulation of CsE1α gene as well as the investigation on the molecular mechanism of cold resistance in pollen of Camellia sinensis.

Key words

Camellia sinensis / cold resistance / promoter / pyruvate dehydrogenase / subcellular localization

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DU Yulin, WANG Weidong, WANG Yuhua, LI Xinghui. Subcellular Localization of CsE1α as well as Cloning and Expression of Its Promoter from the Pollen of Camellia sinensis[J]. Journal of Tea Science. 2015, 35(3): 290-298 https://doi.org/10.13305/j.cnki.jts.2015.03.012

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