茶树花粉中丙酮酸脱氢酶（CsE1α）的定位分析及其启动子的克隆与表达

doi:10.13305/j.cnki.jts.2015.03.012

Abstract

Abstract: In this paper, full-length cDNA is identified for designing the gene-special primers in TAIL-PCR to clone the promoter of CsE1α. By sequencing and bioinformatic analyzing, we observed two CAAT-box, two TATA-box, two GATA-box, one LTR and one G-box located in the 336βbp promoter region. After constructing and transferring the transient expression vectors into the onion epidermal cells, subcellular localization of CsE1α-GFP fusion proteins is verified in mitochondria, while the promoter can activate downstream gene expressing in entire cell. This experiment may provide useful information for the subsequent stable expression in transgenic Arabidopsis and the further investigation on the expression and regulation of CsE1α gene as well as the investigation on the molecular mechanism of cold resistance in pollen of Camellia sinensis.

Key words: Camellia sinensis, pyruvate dehydrogenase, promoter, subcellular localization, cold resistance

CLC Number:

DU Yulin, WANG Weidong, WANG Yuhua, LI Xinghui. Subcellular Localization of CsE1α as well as Cloning and Expression of Its Promoter from the Pollen of Camellia sinensis[J]. Journal of Tea Science, 2015, 35(3): 290-298.

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Subcellular Localization of CsE1α as well as Cloning and Expression of Its Promoter from the Pollen of Camellia sinensis

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