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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (5): 621-630.doi: 10.13305/j.cnki.jts.2023.05.001

• Research Paper • Previous Articles     Next Articles

Catalytic Function, Promoter Structure and Functional Analysis of CsNUDX1-cyto in Different Tea Cultivars

YANG Jihong, ZHOU Hanchen*, XU Yujie   

  1. Tea Research Institute, Anhui Academy of Agricultural Sciences, Huangshan 245000, China
  • Received:2023-08-03 Revised:2023-09-07 Online:2023-10-15 Published:2023-11-06

Abstract: Geraniol is an important monoterpenoid in tea plants, and its accumulation varies greatly among different tea cultivars. The recent study shows that CsNUDX1-cyto is responsible for the production of geraniol and its glycosides in tea plants. In order to explore the differences in the catalytic function and regulation of CsNUDX1-cyto in different tea cultivars, this study analyzed the differences in the accumulation of geraniol and expression patterns of CsNUDX1-cyto, and analyzed the differences in the catalytic function, promoter structure and function of CsNUDX1-cyto in seven tea cultivars. The result shows that CsNUDX1-cyto expression was positively correlated with geraniol content (r=0.805). The content of geranyl in fresh leaves of Camellia sinensis var. sinensis (CSS) was significantly higher than that in C. sinensis var. assamica (CSA) cultivars. Agrobacterium tumefaciens-mediated genetic transformation system shows that CsNUDX1-cyto of different tea cultivars could promote the biosynthesis of geraniol. Analysis of promoter activity shows that CsNUDX1-cyto promoter had the weakest activity in ‘Yunkang 10’, and the structural analysis shows that the promoter of CsNUDX1-cyto in ‘Yunkang 10’ had an 185 base sequence insertion at the transcription start site -33, making the enhancing element CAAT-box located at -133 (CAAT-boxes in other cultivars were located at -47). The results of this study indicate that CsNUDX1-cyto in different tea cultivars could promote geraniol biosynthesis, but the genetic diversity of the promoter region results in differences in its expression level.

Key words: tea plant, geraniol, NUDX1 gene, function analysis, promoter analysis

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