Cloning and Expression Analysis of Nitrate Transporter NRT1.1 Gene in Tea Plant (Camellia sinensis (L.))

YANG Yiyang; HU Yunfei; WAN Qing; LI Ronglin; WANG Feng; RUAN Jianyun

doi:10.13305/j.cnki.jts.2016.05.009

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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5) : 505-512. DOI: 10.13305/j.cnki.jts.2016.05.009

Cloning and Expression Analysis of Nitrate Transporter NRT1.1 Gene in Tea Plant (Camellia sinensis (L.))

YANG Yiyang^1,2, HU Yunfei¹, WAN Qing¹, LI Ronglin¹, WANG Feng³, RUAN Jianyun^2,*

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Abstract

A full length cDNA sequence of Nitrate transport gene (NRT1.1) was obtained from tea plant (Camellia sinensis (L.)) cultivar ‘Longjing 43’ by polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR (RACE-PCR). The length of nucleotide sequence of this gene was 1 880 bp, containing a complete open reading frame (1 788 bp) to encode 595 amino acids. The putative protein had an isoelectric point of 8.99 and a calculated molecular weight of 65.9 kD. CsNRT1.1 was highly homologous to the gene NRT1.1 in Vitis vinifera by sequence alignment. Several parameters of these sequences, including sequences composition, physicochemical property, topological structure of transmembrane regions, hydrophobicity or hydrophilicity, subcellular localization were predicted by bioinformatics tools. Quantitative real-time PCR analysis showed that the expression of CsNRT1.1 in roots and leaves were inhibited after incubation in 1 mol·L^-1 NO₃^- for 5 min. The expressions of CsNRT1.1 in roots were always lower than that of CK within 24 h. Its expressions in leaves were higher than those in roots with its peak

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Camellia sinensis / nitrate / NRT1.1 / RT-PCR

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YANG Yiyang, HU Yunfei, WAN Qing, LI Ronglin, WANG Feng, RUAN Jianyun. Cloning and Expression Analysis of Nitrate Transporter NRT1.1 Gene in Tea Plant (Camellia sinensis (L.))[J]. Journal of Tea Science. 2016, 36(5): 505-512 https://doi.org/10.13305/j.cnki.jts.2016.05.009

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