Transcriptome Analysis of Root Induced by Aluminum in Tea Plants (Camellia sinensis)

HUANG Danjuan; TAN Rongrong; CHEN Xun; WANG Hongjuan; GONG Ziming; WANG Youping; MAO Yingxin

doi:10.13305/j.cnki.jts.2019.05.002

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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (5) : 506-520. DOI: 10.13305/j.cnki.jts.2019.05.002

Transcriptome Analysis of Root Induced by Aluminum in Tea Plants (Camellia sinensis)

HUANG Danjuan¹, TAN Rongrong¹, CHEN Xun¹, WANG Hongjuan¹, GONG Ziming¹, WANG Youping², MAO Yingxin^{1, *}

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Abstract

The aim of this study was to investigate the gene regulation network and expression pattern of the response to aluminum (Al) in tea plants, and to identify the key candidate genes for understanding molecular mechanism of Al tolerance in tea plants. The roots’ antioxidant enzyme activities and Al content of Fuding Dabaicha cultivar were detected under 0βmmol·L^-1, 0.2βmmol·L^-1, 1βmmol·L^-1, 2βmmol·L^-1 and 4βmmol·L^-1 Al³⁺ concentrations for 7βd. The total RNA of roots under 0βmmol·L^-1 (R0), 1βmmol·L^-1 (R1) and 4βmmol·L^-1 (R4) Al³⁺ concentrations were extracted for high-through transcriptome sequencing by Illumina Hiseq Xten platform. The results showed that with the increase of Al concentration, POD activity decreased while APX activity increased gradually. SOD activity reached the highest peak at the Al concentration of 1βmmol·L^-1. However, CAT activity showed no significant difference among five treatments. The Al content first increased and then decreased with the increase of Al³⁺ concentration, and reached the highest peak at the Al³⁺ concentration of 1βmmol·L^-1.The DEGs of R1 VS R0, R4 VS R0, R4 VS R1 were 1β894, 2β439 and 1β384 respectively with 733 (1β161), 846 (1β593) and 628 (756) DEGs significantly up-regulated (down-regulated). GO enrichment analysis shows that the most enrichment biological pathway of three samples were all stimulus responses. In terms of molecular function and cell components, R1 VS R0 and R4 VS R0 were mostly enriched in nucleic acid binding transcription factor activity and cell periphery, while R4 vs R1 were mostly enriched in redox enzyme activity and membrane. KEGG enrichment analysis illustrates that they were significantly enriched in 29, 41, and 19 pathways, respectively, including transcription factors, transporters, plant-pathogen interactions, and phenylpropanoid biosynthesis pathways. It was found that genes involved in physiological processes such as reactive oxygen metabolism, organic acids or metal transporters, transcription factors and cell wall structure modification were up-regulated or inhibited after Al induction, suggesting that these genes were closely related to the molecular mechanism of Al tolerance in tea plants.

Key words

Camellia sinensis / aluminum / differential expressed genes / RNA sequencing / root

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HUANG Danjuan, TAN Rongrong, CHEN Xun, WANG Hongjuan, GONG Ziming, WANG Youping, MAO Yingxin. Transcriptome Analysis of Root Induced by Aluminum in Tea Plants (Camellia sinensis)[J]. Journal of Tea Science. 2019, 39(5): 506-520 https://doi.org/10.13305/j.cnki.jts.2019.05.002

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