ISSR分子标记在茶树遗传关系分析中的初步应用

doi:10.13305/j.cnki.jts.2007.01.007

Abstract

Abstract: Inter-simple sequence repeat (ISSR) is a kind of effective molecular marker developed on the basis of microsatellite, or simple sequence repeat (SSR). ISSR markers were applied to detect the relationship of the thirty-nine cultivars of tea plants. Fifteen primers, selected from fourty primers, were used to amplify the tea samples one hundred and fourty-three DNA bands were amplified, of which one hundred and thirty-one bands were polymorphic.The percentage of polymorphic bands was 91.6%.By cluster analysis based on ISSR markers using UPGMA,the genetic similarity was from 0.21 to 0.95. The GS was the greatest between Manqilan and Zhuyeqilan, and they had the highest genetic similarity and the smallest genetic distance.The GS between Chongqinpipacha and Yinghong 1 was the smallest and they had the lowest genetic similarity and the greatest genetic difference. Cluster analysis classified the 39 cultivars into 3 major groups (GS=0.20). Chongqinpipacha and Yinghong 1 fell into one group, belong to primitive form Jiulongzhu and Huanglong also fell into one group. The rest formed the third group. The results suggest that ISSR is a useful tool for molecular genetic diversity and relationship analysis of tea cultivars.

Key words: ISSR, genetic diversity, relationship, tea plants

CLC Number:

S571.1
Q523

LIN Zheng-he, CHEN Rong-bing, CHEN Chang-song, LIN Jin-ke, HAO Zhi-long, GAO Shui-lian, CHEN Lian-cheng. Preliminary Application of ISSR Markers in the Genetic Relationship Analysis of Tea Plants[J]. Journal of Tea Science, 2007, 27(1): 45-50.

References 15

[1]	Zietkiewica E, Rafalski A, Labuda D.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics 1994, 20: 176~183.
[2]	铁双贵, 郑用琏, 尹艳. 玉米新合成群体8种等位酶17个位点的遗传多样性及与数量性状相关性研究[J]. 作物学报, 1999, 25(6): 759~765.
[3]	程春明, 石云素, 宋燕春, 等. ISSR分子标记技术在分析玉米自交系遗传关系研究中的适用性[J]. 植物遗传资源学, 2005, 6(2): 172~177.
[4]	杨若林, 孔俊, 吴鑫, 等. ISSR标记在辣椒资源遗传多态性分析中的初步应用[J]. 上海大学学报(自然科学版), 2005, 11(4): 235~237
[5]	刘勋成, 李玉媛, 陈少瑜. 不同样树种源遗传多样性的ISSR分析[J]. 西部林业科学, 2005, 34(2): 87~91.
[6]	李莹, 文景芝, 刘春来. 大豆疫霉ISSR-PCR反应体系的建立[J]. 东北农业大学学报, 2005, 36(2): 56~60.
[7]	杨本超, 肖炳光, 陈学军, 等. 基于ISSR标记的烤烟种质遗传多样性研究[J]. 遗传, 2005, 27(5): 753~758.
[8]	佟汉文, 孙群, 吴波, 等. 乌拉尔甘草ISSR-PCR反应体系优化研究[J]. 中国农学通报, 2005, 21(4): 156~159.
[9]	刘威生, 冯晨静, 杨建民, 等. 杏ISSR反应体系的优化和指纹图谱的构建[J]. 果树学报, 2005, 22(6): 626~629.
[10]	姚明哲, 王新超, 陈亮, 等. 茶树ISSR-PCR反应体系的建立[J]. 茶叶科学, 2004, 24(3): 172~176.
[11]	姚明哲, 黄海涛, 余继忠, 等. ISSR在茶树品种分子鉴别和亲缘关系研究中的适用性分析[J]. 茶叶科学, 2005, 25(2): 153~157.
[12]	陈亮, 陈大明, 高其康, 等. 茶树基因组DNA提纯与鉴定[J]. 茶叶科学, 1997, 17(2): 177~181.
[13]	罗军武, 沈程文, 施兆鹏, 等. 茶树基因组DNA提取纯化技术研究[J]. 茶叶通讯, 2002(4): 20~24.
[14]	林郑和, 陈荣冰, 陈常颂. 茶树ISSR-PCR反应体系优化研究[J]. 福建农业学报, 2006, 21(3): 247~252
[15]	杨玉玲, 马祥庆, 张木清. ISSR分子标记及其在树木遗传育种研究中的应用[J]. 亚热带农业研究, 2006(2): 18~23.

Preliminary Application of ISSR Markers in the Genetic Relationship Analysis of Tea Plants

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 15

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City [J]. Journal of Tea Science, 2022, 42(5): 601-609.
[2]	CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features [J]. Journal of Tea Science, 2022, 42(5): 649-660.
[3]	LI Changle, GE Yue, YAN Meilin, LI Hui, LIN Qingqing, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei, NI Dejiang. Analysis of Genetic Diversity and Population Structure of 32 Tea Landraces in China [J]. Journal of Tea Science, 2021, 41(5): 619-630.
[4]	MAO Juan, JIANG Hongjian, YANG Rubing, LI Chongxing, MA Chengying, CHEN Liang, MA Jianqiang. Genetic Diversity and Population Structure of Wild and Cultivated Camellia taliensis Populations [J]. Journal of Tea Science, 2021, 41(4): 454-462.
[5]	LIU Dingding, WANG Junya, TANG Rongjin, CHEN Liang, MA Chunlei. Genome-wide Identification of PPR Gene Family and Expression Analysis of Albino Related Genes in Tea Plants [J]. Journal of Tea Science, 2021, 41(2): 159-172.
[6]	LI Hongjian, QIN Dandan, JIANG Xiaohui, FANG Kaixing, WANG Qiushuang, PAN Chendong, LI Bo, WANG Qing, WU Hualing. Analysis and Evaluation of Purine Alkaloid Contents in Bitter Tea Germplasm Resources from Guangdong [J]. Journal of Tea Science, 2021, 41(1): 71-79.
[7]	HUANG Fangfang, LI Qin, HUANG Jian'an. Research Progress of Tea Rhizosphere Microorganisms [J]. Journal of Tea Science, 2020, 40(6): 715-723.
[8]	SU Jingjing, RUAN Li, WANG Liyuan, WEI Kang, WU Liyun, BAI Peixian, CHENG Hao. Early Identification of Nitrogen Absorption Efficiency in Tea Plants [J]. Journal of Tea Science, 2020, 40(5): 576-587.
[9]	YAN Fei, JIANG Wenhua, QU Dong, FU Jing, ZHAO Xuan. Effects of Exogenous 5-aminolevulinic Acid on Photosynthetic and Physiological Characteristics of Tea Plants under Low Temperature Stress [J]. Journal of Tea Science, 2020, 40(5): 597-606.
[10]	LIU Zhen, CHENG Yang, YANG Peidi, ZHAO Yang, NING Jing, YANG Yang. Genetic Diversity and Structure of Chengbudong Tea Population Revealed by nSSR and cpDNA Markers [J]. Journal of Tea Science, 2020, 40(2): 250-258.
[11]	CHEN Linmu, CHEN Jingguang, WANG Ningning, ZHANG Xianchen. The Role of Plasma Membrane H⁺-ATPase on Nitrogen-regulated Phosphorus Uptake in Tea Plants [J]. Journal of Tea Science, 2019, 39(6): 723-730.
[12]	WANG Feng, SHAN Ruiyang, CHEN Yuzhen, LIN Dongliang, ZANG Chunrong, CHEN Changsong, YOU Zhiming, YU Wenquan. A Case Study of Cadmium Distribution in Soil-Tea Plant-Tea Soup System in Central Fujian Province and Relative Health Risk Assessment [J]. Journal of Tea Science, 2018, 38(5): 537-546.
[13]	WANG Feng, WU Zhidan, CHEN Yuzhen, JIANG Fuying, ZHU Liugang, ZHANG Wenjin, WENG Boqi, YOU Zhiming. Effects of the Combined Application of Biochar and Nitrogen on Growth and Nitrogen Use Efficiency of Tea Plants [J]. Journal of Tea Science, 2018, 38(4): 331-341.
[14]	DING Zhou, LI Yexin, YUAN Yi, WANG Haiyan, LIU Xueshi, JIANG Changjun. Phenotypic Traits and Genetic Diversity of Elite Tea Population in Anhui（China） [J]. Journal of Tea Science, 2018, 38(2): 155-161.
[15]	LIN Weidong, CHEN Zhidan, SUN Weijiang, YANG Ruxing. Analysis of Genetic Diversity of Fujian Tea Varieties by SCoT Markers [J]. Journal of Tea Science, 2018, 38(1): 43-57.