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Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (4): 271-279.doi: 10.13305/j.cnki.jts.2007.04.001

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Genetic Diversity of Ancient Tea Plant in Yunnan Province of China Revealed by Inter-simple Sequence Repeat(ISSR) Polymerase Chain Reaction

JI Peng-zhang1, 2, 3, ZHANG Jun2, *, WANG Ping-sheng2, HUANG Xing-qi3, *, XU Mei2, TANG Yi-chun2, LIANG Ming-zhi2   

  1. 1. College of Life Sciences, Yunnan University, Kunming 650091, China;
    2. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai, 666201, China;
    3. Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
  • Received:2007-04-10 Revised:2007-07-03 Online:2007-12-25 Published:2019-09-11

Abstract: Tea plant [Camellia sinensis var. assamica] populations in Yunnan province of China cultivated since ancient times are important genetic resources for future breeding of new tea cultivars. To evaluate the genetic diversity within and between Yunnan ten representative populations and to formulate conservation strategies for the ancient tea populations in Yunnan was conducted by ISSR technique in this study. The genetic diversity within populations calculated from Nei's genetic diversity (He) was estimated to be 0.281, on average, at the population level and 0.461 at the variety level, while Shannon indices (Ho) of population level and Assam variety level were 0.418 and 0.653, respectively and the percentage of polymorphic loci (P) of the ten populations ranged between 56.5% to 90.9%, respectively. Coefficients of gene differentiation (Gst) within the population was 0.391, in agreement with the result of Shannon’ diversity indices analysis (36.0%) and analysis of molecular variance (AMOVA) among population (39.7%), which illuminated that 60.9% of the variation in Assam variety were from the individuals of the population, and 39.1% of the genetic variation were from the population. The result indicated Camellia var. assamica variety possesses high genetic diversity and medium genetic differentiation was existed in the genetic variation between population. This could be caused by the highly outcrossing nature and habitat fragmentation of tea species. Conservation strategy are suggested including in situ strategies and ex situ strategies based on the observed genetic information of population.

Key words: tea plant (Camellia sinensis var. assamica), ISSR, genetic diversity, conservation strategy

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