Genetic Diversity of Ancient Tea Plant in Yunnan Province of China Revealed by Inter-simple Sequence Repeat(ISSR) Polymerase Chain Reaction

JI Peng-zhang; ZHANG Jun; WANG Ping-sheng; HUANG Xing-qi; XU Mei; TANG Yi-chun; LIANG Ming-zhi

doi:10.13305/j.cnki.jts.2007.04.001

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Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (4) : 271-279. DOI: 10.13305/j.cnki.jts.2007.04.001

Genetic Diversity of Ancient Tea Plant in Yunnan Province of China Revealed by Inter-simple Sequence Repeat(ISSR) Polymerase Chain Reaction

JI Peng-zhang^{1, 2, 3}, ZHANG Jun^{2, *}, WANG Ping-sheng², HUANG Xing-qi^{3, *}, XU Mei², TANG Yi-chun², LIANG Ming-zhi²

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Abstract

Tea plant [Camellia sinensis var. assamica] populations in Yunnan province of China cultivated since ancient times are important genetic resources for future breeding of new tea cultivars. To evaluate the genetic diversity within and between Yunnan ten representative populations and to formulate conservation strategies for the ancient tea populations in Yunnan was conducted by ISSR technique in this study. The genetic diversity within populations calculated from Nei's genetic diversity (He) was estimated to be 0.281, on average, at the population level and 0.461 at the variety level, while Shannon indices (Ho) of population level and Assam variety level were 0.418 and 0.653, respectively and the percentage of polymorphic loci (P) of the ten populations ranged between 56.5% to 90.9%, respectively. Coefficients of gene differentiation (Gst) within the population was 0.391, in agreement with the result of Shannon’ diversity indices analysis (36.0%) and analysis of molecular variance (AMOVA) among population (39.7%), which illuminated that 60.9% of the variation in Assam variety were from the individuals of the population, and 39.1% of the genetic variation were from the population. The result indicated Camellia var. assamica variety possesses high genetic diversity and medium genetic differentiation was existed in the genetic variation between population. This could be caused by the highly outcrossing nature and habitat fragmentation of tea species. Conservation strategy are suggested including in situ strategies and ex situ strategies based on the observed genetic information of population.

Key words

genetic diversity / conservation strategy / ISSR / tea plant (Camellia sinensis var. assamica)

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JI Peng-zhang, ZHANG Jun, WANG Ping-sheng, HUANG Xing-qi, XU Mei, TANG Yi-chun, LIANG Ming-zhi. Genetic Diversity of Ancient Tea Plant in Yunnan Province of China Revealed by Inter-simple Sequence Repeat(ISSR) Polymerase Chain Reaction[J]. Journal of Tea Science. 2007, 27(4): 271-279 https://doi.org/10.13305/j.cnki.jts.2007.04.001

References

[1] Wight W.Nomenclature and classification of tea plant[J]. Nature, 1959, 183: 1726~1728.
[2] Zeng Y W, Wang J J, Yang Z Y, ,et al. The diversity. The diversity and sustainable development of crop genetic resource in the Lancang River Valley [J]. Genet Resour Crop Evol, 2004, 48: 297~306.
[3] Chen J, Wang P S, Xia Y M, et al. Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies[J]. Genet Resour Crop Evol, 2005, 52: 41~52.
[4] 张宏达. 茶叶植物资源的修订[J]. 中山大学学报(自然科学), 1984, 1: 1~12.
[5] 闵天禄. 山茶属茶组植物的修订[J]. 云南植物研究, 1992, 2: 115~132.
[6] 陈亮, 虞富莲, 童启庆. 关于茶组植物分类与演化的讨论[J]. 茶叶科学, 2000, 20(2): 89~94.
[7] 沙丽青, 郭辉军. 云南古茶资源有效保护与合理利用[A]. 中国生态学会. 第二届全国复合生态与循环经济学术讨论会论文集[C]. 北京: 中国科学技术出版社, 2005: 362~365.
[8] 齐丹卉, 郭辉军, 崔景云, 等. 云南澜沧景迈古茶园生态系统植物多样性评价[J]. 生物多样性, 2005, 13(3): 221~231.
[9] 张一平, 刘洋. 云南古茶园与常规茶园小气候特征比较研究[J], 华南农业大学学报, 2005, 26(2): 17~21.
[10] 姚明哲, 黄海涛, 余继忠, 等. ISSR在茶树品种分子鉴别和亲缘关系研究中的适用性分析[J]. 茶叶科学, 2005, 25(2): 133~137.
[11] Doyle J.DNA protocols for plants-CTAB total DNA isolation. In: Hewitt GM, Johnston A, eds. Molecular techniques in taxonomy. Berlin: Springer, 1991, 283~293.
[12] Yeh F C, Yang R C, Boyle T.POPGENE. Microsoft Windows-based freeware for population genetic analysis. Release 1.31[M]. Edmonton: University of Alberta, 1999.
[13] Nei M.Analysis of gene diversity in subdivided populations[J]. Proc. Nat. Acad. Sci. USA, 1973, 70:3321~3323.
[14] Miller M P.Tools for Population Genetic Analysis. Version 1.3[M]. Department of biological sciences, Northern Arizona University, Flag staff. 1997.
[15] Korron J D.A comparison of levels of genetic polymorphism and self-compatibility in geographically restricted and widespread plant congeners[J]. Ecology, 1987, 1: 47~58.
[16] 操国兴, 谢德体, 钟章成, 等. 四川大头茶在不同生境中的遗传分化[J]. 西南农业大学学报, 2003, 25: 200~202.
[17] 操国兴, 钟章成, 谢德体, 等. 缙云山川鄂连蕊茶在不同群落类型中的RAPD分析[J]. 生态学报, 2003, 23: 1583~1589.
[18] 李斌, 尹逸, 周英, 等. 南昆山毛叶茶和云南大叶种的RAPD分子标记研究[J]. 茶叶科学, 2003, 23(2): 146~150.
[19] 罗晓莹,唐光大,许涵,等. 山茶科3种中国特有濒危植物的遗传多样性研究[J]. 生物多样性, 2005, 13(2): 112~121.
[20] 罗军武, 施兆鹏, 沈程文, 等. 茶树种质资源遗传多样性的RAPD分析[J]. 作物学报, 2004, 30(3): 266~269.
[21] 陈亮, 杨亚军, 虞富莲, 等. 15个茶树品种遗传多样性的RAPD分析[J]. 茶叶科学, 1998, 18(1): 21~27.
[22] 金惠淑, 梁月荣, 陆建良. 中、韩两国主要茶树品种基因组DNA多态性比较研究[J]. 茶叶科学, 2001, 21(2): 103~107.
[23] Wachira F N, Powell W. Waugh R.An assessment of genetic diversity among camellia sinensis (cultivated tea)and its wild relatives based on randomly amplified polymorphic DNA and organelle-specific STS[J]. Heredity, 1997, 78: 603~611.
[24] Matsumoto S, Takeuchi A, Hayastsu M, et al. Molecular cloning of phenylalanine ammonia-lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probes[J]. Theor Appl Genet, 1994, 89: 671~675.
[25] Paul S, Wachira F N, Powell W, et al. Diversity and genetic differentiation among population of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers[J]. Theor Appl Genet, 1997, 94: 255~263.
[26] Kaundun S S, Young-Goo Park.Genetic structure of six Korean tea populations as revealed by RAPD-PCR Markers[J]. Crop Sci, 2002, 42: 594~601.
[27] Kaundun S S, Matsumoto S.Heterologous nuclear and chloroplast microsatellite amplification and variation in tea[J]. Camellia sinensis.Genome, 2002, 45: 1041~1048.
[28] Takeda Y.Cross compatibility of tea plant (Camellia sinensis) and its allied species in the Genus Camellia[J]. Jpn. Agric. Res. Q, 1990, 24: 111~116.
[29] Schaal B A, Hayworth D A, Olsen K M, et al. Phylogeographic studies in plants: problems and prospects[J]. Mol Ecol, 1998, 7: 465~474.
[30] Hamrick J L, Godt M J M, Sherman-Broyles S L. Factors influencing levels of genetic in woody plant species[J]. New Forests, 1992, 6: 95~124.
[31] Nybom H. comparion of different nuclear DNA markers for estimating intraspecific genetic diversity in plants[J]. Mol Ecol, 2004, 13: 1143~1155.
[32] Ellstrand N C, Elam D R.Population genetic consequences of small population size: implication for plant conservation[J]. Annu Rev Ecol. Syst, 1993, 24: 217~242.
[33] Wright S.The genetical structure of populations[J]. Annals of Eugenics, 1951, 15: 323~354.
[34] Wallace L E.Examining the effects of fragmentation on genetic variation in Platanthera leucophaea (Orchidaceae): inferences from allozyme and random amplified polymorphic DNA markers[J]. Pl Sp Biol, 2002, 17: 37~49.
[35] Milligan B G, Leebens-Mack J, Strand A E.Conservation genetics: beyond the maintenance of marker diversity[J]. Mol Ecol, 1994, 12: 844~855.