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Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (4): 286-292.doi: 10.13305/j.cnki.jts.2007.04.003

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Preliminary Study on Differential Gene Expression During Cold Acclimation in Tea Plant (Camellia sinensis)

MEI Ju-fen1, 2, WANG Xin-chao2, YANG Ya-jun2, *, LI Xing-hui1, *   

  1. 1. Tea Science Research Constitution of Nanjing Agricultural University, Nanjing 210095, China;
    2. Key Lab of Tea Chemical Engineering, Ministry of Agriculture/Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2007-05-17 Revised:2007-08-20 Online:2007-12-25 Published:2019-09-11

Abstract: Differential gene expression during cold acclimation was studied by mRNA differential display reverse transcription-PCR (DDRT-PCR) in tea plant (Camellia sinensis). Twenty-three differentially expressed cDNA fragments were steadily re-amplified. Five fragments were confirmed by RT-PCR analysis. Among them, three were up-regulated while other two were down-regulated during the cold acclimation process. Sequencing of three differential fragments revealed homology to putative cis-zeatin-O-glucosyl-transferase of Sorghum (Sorghum bicolor), chloroplast gene for chloroplast product of cucumber(Cucumis sativus),ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS) mRNA of tea plant(Camellia sinensis), respectively, while the other two showed no homologous to known genes.

Key words: tea plant (Camellia sinensis), cold acclimation, mRNA differential display reverse transcription-PCR, fragments

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