茶树冷驯化过程中基因表达差异的初步分析

doi:10.13305/j.cnki.jts.2007.04.003

Abstract

Abstract: Differential gene expression during cold acclimation was studied by mRNA differential display reverse transcription-PCR (DDRT-PCR) in tea plant (Camellia sinensis). Twenty-three differentially expressed cDNA fragments were steadily re-amplified. Five fragments were confirmed by RT-PCR analysis. Among them, three were up-regulated while other two were down-regulated during the cold acclimation process. Sequencing of three differential fragments revealed homology to putative cis-zeatin-O-glucosyl-transferase of Sorghum （Sorghum bicolor）, chloroplast gene for chloroplast product of cucumber(Cucumis sativus),ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS) mRNA of tea plant(Camellia sinensis), respectively, while the other two showed no homologous to known genes.

Key words: tea plant (Camellia sinensis), cold acclimation, mRNA differential display reverse transcription-PCR, fragments

CLC Number:

S571.1
Q523

MEI Ju-fen, WANG Xin-chao, YANG Ya-jun, LI Xing-hui. Preliminary Study on Differential Gene Expression During Cold Acclimation in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2007, 27(4): 286-292.

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Preliminary Study on Differential Gene Expression During Cold Acclimation in Tea Plant (Camellia sinensis)

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