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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (3): 331-346.doi: 10.13305/j.cnki.jts.2022.03.003

• Research Paper • Previous Articles     Next Articles

Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis)

WANG Tao1,3,4,5, WANG Yiqing1,3,4,5, QI Siyu1,3,4,5, ZHOU Zhe1,3,4,5, CHEN Zhidan2,3,4,5,*, SUN Weijiang1,3,4,5,*   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Anxi College of Tea Science, Fujian Agriculture and Forestry University, Quanzhou 362400, China;
    3. Engineering Technology and Research Center of Fujian Tea Industry, Fuzhou 350002, China;
    4. Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    5. Tea Industry Technology Development Base of Fujian Province, Fuzhou 350002, China
  • Received:2021-12-14 Revised:2022-01-24 Online:2022-06-15 Published:2022-06-17

Abstract: Chlorophyllase (CLH) is the key enzyme in the degradation of chlorophyll, stripping its phytol to form dephytolithochlorophyll a. The full-length cDNA sequences of three CsCLHs genes were obtained from the second leaves of albino tea cultivar ‘Baijiguan', and bioinformatics analysis was performed. The results show that the three CsCLH genes could be divided into two subfamilies. The full length of CsCLHs was 894-975 bp, encoding 297-324 amino acids. The protein molecular weights were 31.99-34.91 kDa. The isoelectric points were 4.89-7.61, and the instability coefficients were 38.94-48.24. CsCLH1.1 and CsCLH1.2 were unstable proteins, while CsCLH2 was a stable protein. The subcellular localization prediction results of Cell Ploc show that three CsCLH proteins were located in chloroplast, while the results of Wolf Psort show that CsCLH1.1 and CsCLH1.2 were located in cytoplasm and CsCLH2 was located in chloroplast. The qRT-PCR results on the ‘Baijiguan' leaves indicated that expressions of CsCLHs were inhibited by shading treatment and light induced CsCLHs' expressions. Expression pattern analysis of CsCLHs shows that CsCLH1s were highly expressed in the albino cultivars. In addition, it was identified that CsCDF5 could bind to the CsCLH1.1 and CsCLH2 promoters according to the yeast one hybrid system. In conclusion, CsCLHs in albino tea leaves might be involved in chlorophyll degradation and play an important role in the process of albino leaf, which provided a reference for further investigation in the function of the CLH gene family and the albinism of leaves in tea plants.

Key words: Camellia sinensis, chlorophyllase, gene family, sequence analysis, differential expression

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