Fungal Mixed Fermentation for The Production of Lipase and Its Activity Analysis in Galloylated Catechin Hydrolysis

FANG Hongfeng; ZHANG Huixia; WANG Guohong; YANG Minhe

doi:10.13305/j.cnki.jts.2019.01.010

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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (1) : 88-97. DOI: 10.13305/j.cnki.jts.2019.01.010

Fungal Mixed Fermentation for The Production of Lipase and Its Activity Analysis in Galloylated Catechin Hydrolysis

FANG Hongfeng¹, ZHANG Huixia¹, WANG Guohong¹, YANG Minhe^1,2,*

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Abstract

In the process of dark tea manufacture, microbial fermentation leads to the biotransformation of catechins and the decrease of the total polyphenol contents. Up to date, the metabolic pathways and regulatory mechanisms of this transformation process were still unclear. The green tea was fermented by mixed fermentation of Aspergillus cristatum strain PE-1 and A. niger strain PE-4. Lipase activity was detected by a differential culture medium plate test and enzyme activity assay. The results indicate that lipase activities in mixed strain fermentation were stronger than that of signal strain fermentation. In mixed fermentation, a lipase protein with a molecular weight of approximately 37βkDa was obtained by protein separation and purification, which had different biotransformation efficiency for EGCG and ECG. HPLC results show that the lipase had a better substrate selectivity for EGCG than that for ECG. The efficiency of EGCG hydrolysis and the yield of EGC were 94.46% and 63.33%. While the efficiency of ECG hydrolysis and the yield of EC were 15.45% and 4.15%. The biotransformation of EGCG and ECG by lipase would promote the study of the catechin metabolism and the production of monomeric catechins.

Key words

biotransformation / EGCG / galloylated catechins / lipase / mixed fermentation

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FANG Hongfeng, ZHANG Huixia, WANG Guohong, YANG Minhe. Fungal Mixed Fermentation for The Production of Lipase and Its Activity Analysis in Galloylated Catechin Hydrolysis[J]. Journal of Tea Science. 2019, 39(1): 88-97 https://doi.org/10.13305/j.cnki.jts.2019.01.010

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