茶树发根中茶氨酸和儿茶素类含量的分析

doi:10.13305/j.cnki.jts.2006.04.014

茶叶科学 ›› 2006, Vol. 26 ›› Issue (4): 305-309.doi: 10.13305/j.cnki.jts.2006.04.014

茶树发根中茶氨酸和儿茶素类含量的分析

张广辉^{1, 2}, 梁月荣^{1, *}, 陆建良¹, 董俊杰¹

1. 浙江大学茶学系,浙江杭州 310029;
2. 江苏省徐州生物工程学校,江苏徐州 221006

收稿日期:2006-06-20 修回日期:2006-09-08 出版日期:2006-12-25 发布日期:2019-09-11
通讯作者: * 梁月荣, E-mail：yrliang@zju.edu.cn
作者简介:张广辉(1973- ),河南孟津人,在读博士研究生,从事茶树遗传育种与分子生物学研究。
基金资助:
国家自然科学基金（30571192）、浙江省科技项目（2004C32079）

Analysis of Theanine and Catechins in Hairy Roots of Camellia sinensis

ZHANG Guang-hui^{1, 2}, LIANG Yue-rong^{1, *}, LU Jian-liang¹, DONG Jun-jie¹

1. Department of Tea Science, Zhejiang University, Hangzhou 310029, China;
2. Xuzhou Higher Vocational School of Bioengineering, Xuzhou 221006, China

Received:2006-06-20 Revised:2006-09-08 Online:2006-12-25 Published:2019-09-11

摘要/Abstract

摘要： 茶树发根在LG₀培养基和添加1βmg/L IBA的1/2MS培养基上生长迅速,但只在一个培养于LG₀培养基的发根克隆中检测到高含量的茶氨酸,含量达到23.12βmg/g鲜重,高于未转化根的18.77βmg/g鲜重。除茶氨酸外,该发根克隆中也检测到高含量的天门冬氨酸、谷氨酸、天门冬酰胺、谷氨酰胺和精氨酸。发根克隆培养于添加1βmg/L IBA的1/2MS培养基,茶氨酸的含量则显著降低,说明培养基组成也是影响发根中茶氨酸的含量的因素之一。茶树发根中也含有少量的儿茶素类单体,主要为儿茶素和表儿茶素。

关键词: 茶树, 发根培养, 茶氨酸, 儿茶素, HPLC

Abstract: Hairy roots induced by Agrobacterium rhizogenes wild strain 15834 were propagated on LG₀ medium and half MS medium containing 1βmg/L IBA to promote rapid growth. Although rapid growth was obtained in half MS media containing 1βmg/L IBA, high content of theanine (23.12βmg/g fresh weight) was only detected in one hairy root clone cultured on LG₀ medium and higher than the non-transformed root (18.77βmg/g fresh weight). High content of aspartic acid, glutamic acid, glutamine, histidine and arginine were also detected in this hairy root clones. Medium constitute also affected the contents of amino acid in hairy root clone, the content of theanine were reduced in two hairy root clones when they were cultivated on half MS with 1βmg/L IBA. Low content of simple catechins, catechin (C) and epicatechin (EC), were also detected in hairy roots.

Key words: Camellia sinensis, hairy root culture, theanine, catechins, HPLC

中图分类号:

S571.1

张广辉, 梁月荣, 陆建良, 董俊杰. 茶树发根中茶氨酸和儿茶素类含量的分析[J]. 茶叶科学, 2006, 26(4): 305-309. doi: 10.13305/j.cnki.jts.2006.04.014.

ZHANG Guang-hui, LIANG Yue-rong, LU Jian-liang, DONG Jun-jie. Analysis of Theanine and Catechins in Hairy Roots of Camellia sinensis[J]. Journal of Tea Science, 2006, 26(4): 305-309. doi: 10.13305/j.cnki.jts.2006.04.014.

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茶树发根中茶氨酸和儿茶素类含量的分析

Analysis of Theanine and Catechins in Hairy Roots of Camellia sinensis

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