生根剂处理对于茶树嫩枝扦插内源激素水平和繁殖的影响

doi:10.13305/j.cnki.jts.2019.01.002

摘要/Abstract

摘要： 为进一步探究茶树不同嫩度的嫩枝插穗根系和地上部生长适宜的生根剂处理时间及内源激素水平,本试验以3年生台茶12号嫩枝为试验材料,采用酶联免疫吸附测定法（ELISA）分析了嫩梢不同部位IAA、ZR、GA₃和ABA含量,同时研究了生根剂处理时间对插穗茎基部激素含量和茶苗扦插快繁的影响。结果表明,在茶树嫩枝顶部一芽一叶至一芽二叶初展梢（BL1）、半功能叶（L2）、第1片功能叶（L3）及其茎段（S1）、第2片功能叶（L4）及其茎段（S2）、第3片功能叶（L5）及其茎段（S3）、第4片功能叶茎段（S4）中,叶片内IAA和ABA以第2片功能叶含量最高,ZR以第3片功能叶含量最高,GA₃含量则随叶片成熟度增加而增大。茎段S1—S3中IAA含量显著高于S4,而ABA含量则为S1显著高于S2—S4。浸泡100βmg·L^-1 ABT-1号生根剂会显著提高插穗茎基部IAA含量,并使IAA/ABA和(IAA+ZR+GA₃)/ABA值增至1.0左右,从而有利于生根。其中,保留顶梢带1片和2片功能叶的插穗（F1-1、F2-1）在浸泡生根剂前期1βh内IAA含量快速增加,且IAA/ABA和(IAA+ZR+GA₃)/ABA值可增至1.0以上,其他插穗则在浸泡生根剂4βh后上述3个指标达到最大值。F1-1、F2-1浸泡生根剂0.5~1βh,摘除顶梢带1片功能叶的插穗（F1-2）浸泡生根剂4βh,F2-2、F3-1、F3-2浸泡生根剂3~4βh,IAA含量达到较高水平,而且IAA/ABA和 (IAA+ZR+GA₃) /ABA值可增至1.0左右。出苗时,平均根系活力、平均生根条数和平均茎粗增加值的最大值出现在浸泡生根剂0~2βh内,其他根系和地上部生长情况以浸泡生根剂4βh表现较好。

关键词: 茶树, 嫩枝扦插, 激素, 快繁生根, 地上部生长

Abstract: In order to explore the appropriate treatment of rooting agent and endogenous hormone changes in softwood cuttings of three-year-old Camellia sinensis (L.) O. Kuntze cv. Taicha12 with different tenderness, twigs were used to investigate the contents of IAA, ZR, GA3 and ABA in different tissues by Enzyme-linked Immunosorbent Assay (ELISA). And the effects of soaking duration of rooting agent on the endogenous hormone contents in the basal stem and the rapid propagation of tea cuttings were also studied. The results showed that in one bud and one leaf to one bud and two leaves (BL1), semi-functional leaf (L2), the first functional leaf (L3) and itslinked stem (S1), the second functional leaf (L4) and its linked stem (S2), the third functional leaf (L5) and its linked stem (S3), the stem near the fourth functional leaf (S4), the highest contents of IAA and ABA were observed in the second functional leaves. The highest content of ZR was found in the third functional leaves. And the content of GA3 increased with the increase of leaf maturity. The contents of IAA in S1-S3 were significantly higher than that in S4. However, the content of ABA in S1 was significantly higher than those in S2-S4. After ABT-1 rooting agent treatment (100 mg·L-1), the content of IAA in the basal stem significantly increased and the IAA/ABA and (IAA+ZR+GA3)/ABA values increased to about 1.0, which facilitated the formation of roots. During one hour treatment of the rooting agent, the IAA content rapidly increased and the IAA/ABA and (IAA+ZR+GA3)/ABA values also increased to about 1.0 in the cuttings with one or two functional leaves and apical shoot (F1-1, F2-1). For other cuttings, the above three indicators reached the maximum value after treatment for 4 h. The best treatment duration for different cuttings were different, with F1-1, F2-1 for 0.5-1 h, the cuttings with one functional leaf and excised apical shoot (F1-2) for 4 h, F2-2, F3-1 and F3-2 for 3-4 h. During transplantation, the maximum values of the root vigor, root number and the increase of basal stem diameter occurred in rooting agent treatment for 0-2 h. Other roots and shoots of tea cuttings showed the best growth after 4 h treatment.

Key words: tea tree, softwood cutting, phytohormone, rapid propagation and rooting, aerial growth

中图分类号:

任恒泽, 张丽霞, 向勤锃, 韩晓阳, 于谦, 蔡鲁. 生根剂处理对于茶树嫩枝扦插内源激素水平和繁殖的影响[J]. 茶叶科学, 2019, 39(1): 11-12. doi: 10.13305/j.cnki.jts.2019.01.002.

REN Hengze, ZHANG Lixia, XIANG Qinzeng, HAN Xiaoyang, YU Qian, CAI Lu. Effects of Rooting Agent on Endogenous Hormone Levels and Propagation of Softwood Cuttings of Tea Plant[J]. Journal of Tea Science, 2019, 39(1): 11-12. doi: 10.13305/j.cnki.jts.2019.01.002.

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