表没食子儿茶素没食子酸酯增强卡莫司汀抗肿瘤作用的体外研究

doi:10.13305/j.cnki.jts.2007.04.007

Abstract

Abstract: The effect of (-)-epigallocatechin gallate (EGCG) combined with carmustine on the growth inhibition and DNA damage of human lung carcinoma cell line A549 were evaluated by MTT assay and Comet assay repectively. The results showed that 50% inhibitory concentration (IC₅₀) of carmustine to A549 human lung carcinoma cell line was decreased when combined with EGCG. The 50% inhibitory concentration (IC₅₀) of carmustine alone to human lung carcinoma cell line A549 was 187.46βμg/ml, When the carmustine is combine with EGCG(25βμg/ml and 50βμg/ml, the concentration under which EGCG has no growth inhibition to A549), however, the IC₅₀ value to A549 cell line decreased to 139.56βμg/ml and 154.02βμg/ml respectively. EGCG also enhanced carmustine-induced DNA damage in A549 cell line by Comet assay. The tail moment increased from 19.02±14.60 to 33.07±10.47. So, EGCG can enhance carmustine-induced DNA damage in A549 cell line, hence enhance the anticancer activity of carmustine.

Key words: EGCG, carmustine, DNA damage, comet assay, IC₅₀

CLC Number:

HUANG Xiu-hua, ZHANG Dan, HAO Jin, YANG Zu-jun, REN Zheng-long. (-)-epigallocatechin Gallate (EGCG) Enhance the Anticancer Activity Induced by Carmustine in vitro[J]. Journal of Tea Science, 2007, 27(4): 302-306.

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(-)-epigallocatechin Gallate (EGCG) Enhance the Anticancer Activity Induced by Carmustine in vitro

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