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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (2): 201-209.doi: 10.13305/j.cnki.jts.2016.02.012

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Inhibition of Biofilm Development and Spoilage Potential in Shewanella baltica by Epigallocatechin Gallate

YE Xiaofeng, ZHANG Fang, ZHU Junli*, ZHANG Lei, XIE Dumei   

  1. College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China
  • Received:2015-11-23 Online:2016-04-15 Published:2019-08-23

Abstract: The anti-biofilm activity of catechin monomers from tea polyphenols against S. baltica, as the specific spoilage organism in marine product, was investigated. Effects of catechin monomer with the highest inhibitory activity on biofilm formation and spoilage potential of S. baltica were further assessed. The results showed that all five monomer of catechins significantly repressed biofilm formation of S. baltica, in which epigallocatechin gallate (EGCG) exhibited the highest activity. The minimum inhibitory concentration (MIC) of EGCG against S. baltica was 40 µg·mL-1, and EGCG at the sub-inhibitory concentration of 10-30 µg·mL-1 had no effect on its growth. EGCG at 30 µg·mL-1 inhibited significantly the activity of quorum sensing (QS) autoinducers, AI-2, cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe) (P<0.05), with reduction by 63.95%, 27.43% and 21.94% respectively. The biofilm development, swimming motility and protease activity also decreased considerably after incubation with sub-MIC of EGCG, and exhibited a dose dependent effect, which presented the reduction by 55.18%, 66.71% and 46.67% in the presence of 30 μg·mL-1 EGCG. Hence, our results indicated that the sub-MIC of EGCG could interfere with S. baltica QS system, and inhibit the biofilm formation and spoilage potential of S. baltica. The present study suggested the potential of EGCG as QS inhibitor in controlling food spoilage bacterial.

Key words: Shewanella baltica, EGCG, biofilm, spoilage, quorum sensing, inhibition

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