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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (3): 236-242.doi: 10.13305/j.cnki.jts.2009.3.010

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Genetic Diversity and Relationship of Huangjincha Cultivar Based on EST-SSR Markers

YANG Yang, LIU Zhen, ZHAO Yang, LIANG Guo-qiang, ZHAO Xi   

  1. Tea Research Institute, Hunan Academy of Agriculture Science, Changsha, 410125, China
  • Received:2008-11-24 Revised:2009-02-25 Online:2009-06-15 Published:2019-09-06

Abstract: The 19 pairs of EST-SSR primers were used to analyze the genetic polymorphism and genetic relationship of 38 Huangjincha individuals. Totally 37 alleles were amplified using 19 EST-SSR primers, the number of alleles per primer ranged from 1 to 3, on average of 1.95. The expected heterozygosity (He) varied from 0.03 to 0.65, and the observed heterozygosity (Ho) ranged from 0 to 0.97, on average of 0.32 and 0.33, respectively. The Shannon index of the Huangjincha was 0.55. All of the results showed that the genetic polymorphism of Huangjincha was relatively poor. The 38 individuals were classified into 6 groups based on the UPGMA method with the similarity coefficient at 0.77. The results of the similarity coefficient among different individuals can give some implications to the genetic improvement of Huangjincha. At the same time, analysis of molecular variance (AMOVA) showed that variance component among groups and within populations were 21.77% and 78.23% each other, gene flow among the groups is 1.80, which all can give us some supports to protect the Huangjincha.

Key words: Huangjincha, EST-SSR, genetic polymorphism, genetic relationship

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