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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (4): 382-391.doi: 10.13305/j.cnki.jts.2019.04.003

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Functional Analysis of Glutathione Peroxidase Encoding Gene CsGPX1 in Camellia sinensis

LIU Sai1,2, LIU Shuoqian1,2,3, LONG Jinhua1,2, WU Dunchao1,2, CHEN Yuhong1,2, LIU Liping1,2, LIU Zhonghua1,2,3, TIAN Na1,2,3,*   

  1. 1. College of Horticulture and Hardening, Hunan Agricultural University, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China;
    3. Key Lab of Tea Science, Ministry of Education, Changsha 410128, China.
  • Received:2019-03-08 Online:2019-08-15 Published:2019-08-19

Abstract: To clarify function of glutathione peroxidase-encoding gene CsGPX1 in Camellia sinensis (L.) O. Kuntze, the full-length sequence of the coding region of CsGPX1 was obtained by transcriptome data of tea plant, and sequence analysis and function prediction were performed. Then, the CsGPX1 was overexpressed in tobacco. Subsequently, the difference in drought tolerance between wild-type and transgenic tobacco was compared to verify the function of CsGPX1. Sequence analysis indicates that CsGPX1 was 723 bp in length, encoding 240 amino acids. The Blast alignment reveals that the GPX showed more than 85% of identity in amino acid with GPX from Prunus persica. The amino acid sequence encoded by CsGPX1 has a conserved characteristic sequence of the GPX protein and a phospholipid hydrogen glutathione peroxidase (PHGPX) which is unique to other family members. The results of drought stress treatment show that the drought resistance of the over-expressing of CsGPX1 in tobacco lines was stronger than that of wild-type. The results of GPX enzyme activity assay show that the GPX enzyme activity of the transgenic plants was higher than that of the wild type plants. All of above results verify that the over-expression of CsGPX1 improved the drought tolerance of transgenic tobacco, suggesting that CsGPX1 might be related to the drought resistance in tea plants. This study could provide a new strategy to improve the drought resistance of tea plants, which would significantly reduce the management cost of tea plantation.

Key words: Camellia sinensis, glutathione, peroxidase, abiotic stress, drought resistance mechanism

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