Determination of Melamine in Milk Tea By High Performance Liquid Chromatography

LIU Xiao-hui; YAO Yu; JIANG He-yuan; YUAN Xin-yue; ZHANG Jian-yong; CUI Hong-chun

doi:10.13305/j.cnki.jts.2009.1.003

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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (1) : 15-19. DOI: 10.13305/j.cnki.jts.2009.1.003

Determination of Melamine in Milk Tea By High Performance Liquid Chromatography

JIANG He-yuan¹, YUAN Xin-yue^1,2, ZHANG Jian-yong¹, CUI Hong-chun^1,2, LIU Xiao-hui^1,2, YAO Yu^1,2

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Abstract

The method to determinate melamine in milk tea by HPLC, was established. The samples were analyzed by a Sunfire C₁₈ column （250 mm×4.6 mm ID; 5 μm）. The mobile phase was composed of 0.008 mol/L sodium 1-Octanesulfonate （adjust to pH3.3 with acetic acid） and acetonitrile. The flow rate was 1.0 ml/min. Column temperature was 30℃. UV-detection was performed at 236 nm. The contents were quantified with exterior standard. Some milk tea samples from the supermarket were analyzed, and results showed that the content of melamine in some milk tea were higher than 2.5 mg/kg, the set limit in national standard.

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HPLC / melamine / milk tea

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JIANG He-yuan, YUAN Xin-yue, ZHANG Jian-yong, CUI Hong-chun, LIU Xiao-hui, YAO Yu. Determination of Melamine in Milk Tea By High Performance Liquid Chromatography[J]. Journal of Tea Science. 2009, 29(1): 15-19 https://doi.org/10.13305/j.cnki.jts.2009.1.003

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