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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (1): 15-19.doi: 10.13305/j.cnki.jts.2009.1.003

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Determination of Melamine in Milk Tea By High Performance Liquid Chromatography

JIANG He-yuan1, YUAN Xin-yue1,2, ZHANG Jian-yong1, CUI Hong-chun1,2, LIU Xiao-hui1,2, YAO Yu1,2   

  1. 1. Key Laboratory of Tea and Beverage Crops Processing and Quality Control, Tea Research Institute, Chinese Academy of Agriculture Sciences, Hangzhou 310008, China;
    2. Graduate School, Chinese Academy of Agriculture Sciences, Beijing 100081, China
  • Received:2008-10-28 Revised:2008-11-27 Online:2009-02-15 Published:2019-09-06

Abstract: The method to determinate melamine in milk tea by HPLC, was established. The samples were analyzed by a Sunfire C18 column (250 mm×4.6 mm ID; 5 μm). The mobile phase was composed of 0.008 mol/L sodium 1-Octanesulfonate (adjust to pH3.3 with acetic acid) and acetonitrile. The flow rate was 1.0 ml/min. Column temperature was 30℃. UV-detection was performed at 236 nm. The contents were quantified with exterior standard. Some milk tea samples from the supermarket were analyzed, and results showed that the content of melamine in some milk tea were higher than 2.5 mg/kg, the set limit in national standard.

Key words: HPLC, milk tea, melamine

CLC Number: