茶树修剪物对茶园土壤真菌群落多样性影响研究

doi:10.13305/j.cnki.jts.2015.03.008

Abstract

Abstract: Returning of pruning material to the field is a common agronomic practice performed in tea plantation. By extracting the total DNA of soil in various treatments, the effects of tea pruning material on fungal community structure and diversity in tea garden soils were investigated by using PCR amplification and denaturing gradient gel electrophoresis (DGGE), the original soil without any supplement was set as control. Many common bands were detected from each soil samples by DGGE, and those bands that showed highest sequence homology to Pezizomycotina, Basidiomycota, Mortierellomycotina and Mucoromycotina. The number and brightness of bands from DGGE presented the community diversity of fungi under different treatments. The results showed the richness and Shannon index were lower than the control, maybe the degradation products and phenolic compounds of pruning material have some inhibitory effect on fungi community diversity.

Key words: tea plant pruning material, PCR-DGGE, community structure, diversity

CLC Number:

YUAN Saiyan, CHEN Xuan, FANG Wanping, WANG Yuhua, ZHU Xujun, LI Xinghui. Effect of Tea Plant Pruning Material on Fungi Community Diversity in Tea Plantation Soil[J]. Journal of Tea Science, 2015, 35(3): 255-262.

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Effect of Tea Plant Pruning Material on Fungi Community Diversity in Tea Plantation Soil

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